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Cell electroporation chip using multiple electric field zones in a single channel

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3 Author(s)
Kim, Min-Ji ; Cell Bench Research Center, Korea Advanced Institute of Science and Technology (KAIST), Daejeon, South Korea ; Kim, Taeyoon ; Cho, Young-Ho

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We present cell electroporation chip using a single channel with multiple electric field, E, zones. The present chip, where multiple E zones are generated by a pair of external electrodes across a stepwise single channel, provides the optimal E conditions for stable cell electroporation with high viability in a single experiment. The optimal E for both H23 and A549 cells was 0.4 kV/cm with the maximum percentage of the viable and electroporated cells of 51.4 ± 3.0% and 26.6 ± 0.7%, respectively. The present cell electroporation chip has potential for use in integrated cell chips to find the optimal E conditions for the electroporation study.

Published in:

Applied Physics Letters  (Volume:101 ,  Issue: 22 )

Date of Publication:

Nov 2012

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