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We present cell electroporation chip using a single channel with multiple electric field, E, zones. The present chip, where multiple E zones are generated by a pair of external electrodes across a stepwise single channel, provides the optimal E conditions for stable cell electroporation with high viability in a single experiment. The optimal E for both H23 and A549 cells was 0.4 kV/cm with the maximum percentage of the viable and electroporated cells of 51.4 ± 3.0% and 26.6 ± 0.7%, respectively. The present cell electroporation chip has potential for use in integrated cell chips to find the optimal E conditions for the electroporation study.
Published in:
Applied Physics Letters
(Volume:101
,
Issue:
22
)
Date of Publication: Nov 2012
- Page(s):
- 223705 - 223705-4
- ISSN :
- 0003-6951
- Digital Object Identifier :
- 10.1063/1.4769037
- Product Type:
- Journals & Magazines
- Date of Current Version :
- 03 December 2012
- Issue Date :
- Nov 2012
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