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An interconnect-driven layout-aware multiple scan tree (MST) synthesis methodology for 3-D integrated circuits (ICs) is proposed. MSTs, also known as scan forest, greatly reduce test data volume and test application time in system-on-a-chip testing. Previous studies on layout-aware scan tree synthesis only address 2-D layouts, so they cannot be directly applied to 3-D ICs. The proposed algorithm effectively optimizes both test compression rate and routing length under 3-D IC-induced constraints, and produces better results than all previous known methods.