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There are many occasions we need to test its temperature in real time, such as, grain storage, boilers, etc. The design is mainly studied the establishment and application of multi-channel temperature measurement system. Besides, it has chosen the STC89C52 as the core control chip, and the DS18B20 chips as sensors, also uses Shift register that called 74HC595 to drive the LED, and uses the chip LM7805 to make the voltage of system more stable. Finally, uses the MAX232 chip to convert the level, and to download the program to the microcontroller. The system is so rapid, accurate, reliable, economic, simple that finally realized the multi-channel temperature measurement. The results show that by laboratory test. The temperature ranges from - 55 ° C to 125 ° C, In - 10 ~ + 85 ° C range, the precision of temperature is ±0.5°C. The system is suitable for common occasions of multichannel temperature measure, has great potential for development and practical significance.