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With the development of embedded systems and system on chip, C51 series of single-chip has entered a new stage. The MCU Module has been added to the latest version of Multisim10, so it can be simulated and analyzed. A practical example is used to illustrate the basic operation process of Multisim10 MCU simulation module using C language by the flowing water light experimental studies in which 8051 single chip was taken as the control core. At the same time, the MCU system design can be proved feasibly. It will be of guidance significance for the development and application of embedded system.