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In this paper, we carried out an experimental study of graphene-on-gold structure based on Surface Plasmon Resonance Imaging (SPRI) method. To our knowledge, this is the first experimental report of graphene SPR study. An experimental SPRI platform was setup, which can fulfill wide incident angle range covering the resonant angle of gas and aqueous sample. 1, 2, 3 layers of graphene are fabricated by CVD method and then transfer to gold substrate. The SPR images of different graphene-on-gold structure are acquired and analyzed. SPR curves of different layers of graphene-on-gold are obtained and compared with simulation result. It is found that the SPR angle will shift about 0.18(deg) per graphene layer, which is consistent with the simulation result (0.15 deg). The graphene-on-gold structure provide an accurate single-atom-layer tool, which can be applied in various research areas, e.g. biosensor, nanoplasmonic etc.