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MANET is an infrastructure-less network formed with the help of mobile nodes carrying wireless devices capable of communicating each other. MANET has dynamic topology due to node mobility. Mobility models define the movement of mobile nodes with respect to location, velocity and acceleration in MANET. Transmissions of Packets between the mobile devices are controlled by Routing Protocols. Simulation is the best tool for performance evaluation of MANET. Performance of routing protocol is affected by mobility models used in the simulation. Mobility Models that are used commonly are either non realistic (Random Waypoint) or Semi-realistic (Manhattan & Reference Point Group Mobility). In this paper, a realistic mobility model has been developed by giving the real time observation of the mobile nodes movement in a realistic health care environment as an input to the simulation. The aim is to determine the performance measures like throughput, packet delivery ratio, delay and protocol overhead that are commonly used in MANET for evaluating ad-hoc routing protocols- DSDV and AODV with realistic mobility model. The routing protocols under realistic mobility model provide higher Throughput, Packet Delivery Ratio and lower Normalized Routing Overhead & Average Delay. AODV routing protocol performs better than DSDV Routing protocol.