Scheduled System Maintenance:
Some services will be unavailable Sunday, March 29th through Monday, March 30th. We apologize for the inconvenience.
By Topic

Influences of oxLDL on ABCA1 mRNA expression and cholesterol outflow rate in U937 cells

Sign In

Cookies must be enabled to login.After enabling cookies , please use refresh or reload or ctrl+f5 on the browser for the login options.

Formats Non-Member Member
$31 $13
Learn how you can qualify for the best price for this item!
Become an IEEE Member or Subscribe to
IEEE Xplore for exclusive pricing!
close button

puzzle piece

IEEE membership options for an individual and IEEE Xplore subscriptions for an organization offer the most affordable access to essential journal articles, conference papers, standards, eBooks, and eLearning courses.

Learn more about:

IEEE membership

IEEE Xplore subscriptions

2 Author(s)
Su Xian-ming ; Dept. of Geriatric Cardiology, Xi'an Jiaotong Univ., Xi'an, China ; Liu Jing-wei

To investigate the oxidized low density lipoprotein (oxLDL) on U937 cell ATP-binding cassette transporter A1 (ABCA1) mRNA expression and cholesterol efflux situation. Human U937 cells were incubated with gradient concentrations of oxLDL(0, 25, 50, 75, 100, 125mg/L), and then dyed by oil red O to estimate the content of intracelluar lipid and detect the expressing quantity of ABCA1 mRNA by Real-time Fluorescence quantitative PCR simultaneously. Calculating the cholesterol efflux rates by using the scintillation counter to detect the amount of H3-cholesterol in each well cell culture plate and medium. Real-time Fluorescence quantitative PCR analysis showed that the expression levels of ABCA1 mRNA in monocytes were lower than basal line when not intervened with oxLDL, and increased drastically with oxLDL stimulation, significant difference compared with controls(P<;0.01), and reached the highest level at oxLDL 50mg/L, nevertheless, continously increasing the concentration of oxLDL above 50mg/L, the expression decreased. So is the outflowing rate of intracelluar lipid. Oil red O dyeing results also suggested that celluar lipid content was the highest when intervened with 125mg/L oxLDL, and increased most obviously at 50mg/L oxLDL. Cholesterol outflow result also demonstrated that cholesterol outflow rate related with the ABCA1 mRNA expressing quantity. With the increase of intervening concentration of oxLDL on U937cells, the exprssion of ABCAl mRNA represented that rising befor 50mg/L oxLDL, and then decreasing, reaching the top point at 50mg/L oxLDL. so was the change in the outflowing rate of intracelluar lipid.

Published in:

Information Technology in Medicine and Education (ITME), 2012 International Symposium on  (Volume:2 )

Date of Conference:

3-5 Aug. 2012