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Nanostructured anatase-titanium dioxide based platform for application to microfluidics cholesterol biosensor

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6 Author(s)
Azahar Ali, Md. ; Department of Science and Technology Centre on Biomolecular Electronics, Biomedical Instrumentation Section, National Physical Laboratory, New Delhi-110012, India ; Srivastava, Saurabh ; Solanki, Pratima R. ; Agrawal, Ved Varun
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We report results of studies relating to the fabrication of a microfluidics cholesterol sensor based on nanocrystalline anatase-titanium dioxide (ant-TiO2) film deposited onto indium tin oxide (ITO) glass. The results of response studies (optimized under the flow rate of 30 μl/min) conducted on cholesterol oxidase (ChOx) immobilized onto crystalline ant-TiO2 nanoparticles (∼27 nm)/ITO microfluidics electrode reveal linearity as 1.3 to 10.3 mM and improved sensitivity of 94.65 μA/mM/cm2. The observed low value of Km (0.14 mM) indicates high affinity of ChOx to cholesterol. No significant changes in current response of this microfluidics sensor are measured in the presence of different interferents.

Published in:

Applied Physics Letters  (Volume:101 ,  Issue: 8 )

Date of Publication:

Aug 2012

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