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Based on the medicine image characteristic, this paper has made the related improvement to the insufficiency which exists in view of its image mosaic algorithm. This paper has designed one kind of image mosaic algorithm which has quick speed, high precision and strong robustness. This paper has studied image mosaic algorithm based on the regional characteristics matching, through extracting image characteristic and using fuzzy set identification mode to find the similar region, it can definite matching mosaic image. The experimental result has indicated that the algorithm proposed in the article can complete the medicine image mosaic precisely and quickly, which has achieved satisfactory mosaic effect.