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Besides the stress around Cu TSV's, also the stress induced by microbumps is a main contributor to transistor level stress. For complete and successful deployment of 3D IC all effects generating stress have to be addressed. Therefore, this work quantifies the stress and its effects associated with Cu microbumps and their interaction with underfill material in 3D stacks by using a combined experimental and theoretical approach. We report on the stress generated by backside microbumps affecting FETs through the thinned silicon die and the stress on the thin die caused by 3D stacking. We find that the FET current shifts reach over 40% due to the impact of stress. Additionaly, a FEM parametric study was performed to determine key stress reduction contributors in 3D stacks.