By Topic

The Evaluation of Cell Respiratory Effect of Magnetic Nanoparticles Using Viability Assay and Chronoamperometric Method

Sign In

Cookies must be enabled to login.After enabling cookies , please use refresh or reload or ctrl+f5 on the browser for the login options.

Formats Non-Member Member
$33 $13
Learn how you can qualify for the best price for this item!
Become an IEEE Member or Subscribe to
IEEE Xplore for exclusive pricing!
close button

puzzle piece

IEEE membership options for an individual and IEEE Xplore subscriptions for an organization offer the most affordable access to essential journal articles, conference papers, standards, eBooks, and eLearning courses.

Learn more about:

IEEE membership

IEEE Xplore subscriptions

4 Author(s)
H. Y. Tsai ; Dept. of Appl. Chem., Chung Shan Med. Univ., Taichung, Taiwan ; S. H. Tsai ; H. W. Deng ; C. B. Fuh

We evaluated the respiratory effect of Fe3O4@polyacrylamide magnetic nanoparticles using both 3-(4, 5-Dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide (MTT) viability assay and the chronoamperometric method, based on screen-printed carbon electrodes (SPE). Preliminary results show that nanoparticles have no significant effect on the respiration of rat liver cells from these two detection methods. The chronoamperometric method using SPE shows that the current responses are proportional to the Ferro cyanide concentrations, with a linear range of 0.03~ 1.0 mM (R2=0.9923), and detection limit (S/N=3) at 32 μM. The results of paired t-test analysis indicate that assessment of hepatocyte viabilities based on the chronoamperometric method were comparable to those of the MTT viability assay. The chronoamperometric method can be used as a quick alternative method for assessing liver-cell viability.

Published in:

Biomedical Engineering and Biotechnology (iCBEB), 2012 International Conference on

Date of Conference:

28-30 May 2012