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Computational optical sectioning microscopy using an engineered PSF with reduced depth variability — Proof of concept

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2 Author(s)
Shuai Yuan ; Department of Electrical and Computer Engineering, the University of Memphis, Memphis ; Chrysanthe Preza

In this simulation study, an engineered point-spread function (PSF) with reduced depth variability (due to depth-induced aberrations) was applied to three-dimensional computational optical-sectioning microscopy (COSM) imaging to investigate its impact on image restoration. Intermediate synthetic images from PSF-engineered COSM in the presence of aberrations were computed and then processed using a depth-variant expectation maximization algorithm. The restored images were compared to images simulated for traditional COSM. Results show that PSF-engineered COSM achieves better image restoration than conventional COSM and has the potential to improve imaging in the presence of depth-induced aberrations.

Published in:

2012 9th IEEE International Symposium on Biomedical Imaging (ISBI)

Date of Conference:

2-5 May 2012