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Lifetime estimation of moving vesicles in frequency-domain fluorescence lifetime imaging microscopy

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3 Author(s)
Roudot, P. ; Inria Rennes - Bretagne Atlantique, Rennes, France ; Kervrann, C. ; Waharte, F.

We propose a robust statistical framework for correcting the movements of vesicles in frequency domain FLIM imaging. Movement and lifetime are jointly estimated in a three-step procedure. Robust M-estimators are mainly used to improve accuracy in temporally-varying noisy images. The performance of the proposed method is demonstrated on both simulated and real samples.

Published in:

Biomedical Imaging (ISBI), 2012 9th IEEE International Symposium on

Date of Conference:

2-5 May 2012