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In this paper we discuss under which conditions standard stereo visual acuity tests can be implemented on 3D TV monitors. In particular, we emphasize the role of environmental lighting conditions, on the measurement of the stereo visual acuity, when using conventional Stereoscopic 3D tests, such as Wirt stereotests. We investigate the impact of parameters such as luminance, backlight and contrast when these tests are implemented on 3D TV monitors. We demonstrate that some deviations are observed when modifying the room luminance and the type of displays used (e.g. plasma (PDP) or liquid crystal (LCD) displays). Our measurements carried out on an human sample are supervised by pupil size measurements, using an eyes-tracker, enabling a better interpretation of the results. Finally, we discuss the benefit of using Stereoscopic 3D tools to implement stereo visual acuity measurements.