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We present a new design of a portable nitrogen cryostat for operation of moving SQUIDs. A mixture of liquid and gaseous nitrogen fills a reservoir in direct contact with a copper part for the SQUID integration. The temperature at the SQUID position is 77.8 K or 78.8 K depending on orientation, and varies within /spl plusmn/10 mK during lateral movement. The cryostat can operate as a portable system for 7 hours without refilling. Washer rf SQUIDs and dc gradiometers were integrated with the cryostat. We proved the operation of the system as a moving magnetometer in an unshielded laboratory environment. Noise spectra in shielding and outside were independent of orientation. The system was equipped with a differential eddy current excitation. We show the first non-destructive material evaluation results for fatigue crack detection on stationary samples with moving SQUID sensors.