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In this paper, L-band microwave observations were assimilated using the ensemble Kalman filter to improve root-zone soil moisture (RZSM) estimates from a coupled soil vegetation atmosphere transfer (SVAT)-vegetation model linked to a forward microwave model. Simultaneous state-parameter updates were performed by assimilating both synthetic and field observations during a growing season of sweet corn every three days, matching the temporal coverage of observations from the Soil Moisture and Ocean Salinity and Soil Moisture Active Passive missions. The sensitivities of parameters to the states were investigated using the information-theoretic measure of conditional entropy. Among the soil parameters, the pore-size index (λ) was the most sensitive to brightness temperatures (TB) during the early and midgrowth stages, while porosity (φ) was the most sensitive to TB during the reproductive stage. In the microwave model, the soil roughness parameters, root mean square (RMS) height (r), and correlation length (l) were the most sensitive during the early and mid stages, while the vegetation regression parameter (b) was the most sensitive during the reproductive stage. In the synthetic experiment, assimilation of TB provided RMS error reductions in RZSM estimates of 70% compared to open loop estimates. Minimal variations in performance were observed across different stages of the season during the synthetic experiment. However, when field observations of TB were assimilated, significant differences in RZSM estimates were observed during different growth stages. Maximum RMS difference (RMSD) reductions in RZSM estimates of 33.3% were observed compared to open loop estimates during the early stages, while improvements of 4.8% and 16.7% were observed in the mid- and reproductive stages, respectively. Further analyses of assimilation with field observations also suggest - ome improvements in the SVAT model are needed for moisture transport immediately following the precipitation/irrigation events. In the microwave model, the linear vegetation formulation for estimating canopy opacity, parameterized by b, was inadequate in capturing the complexities in TB during stages of high vegetative and reproductive growth rates.