By Topic

Microfluidic Platform for Electrical Monitoring of Enzyme Activity

Sign In

Cookies must be enabled to login.After enabling cookies , please use refresh or reload or ctrl+f5 on the browser for the login options.

Formats Non-Member Member
$31 $13
Learn how you can qualify for the best price for this item!
Become an IEEE Member or Subscribe to
IEEE Xplore for exclusive pricing!
close button

puzzle piece

IEEE membership options for an individual and IEEE Xplore subscriptions for an organization offer the most affordable access to essential journal articles, conference papers, standards, eBooks, and eLearning courses.

Learn more about:

IEEE membership

IEEE Xplore subscriptions

2 Author(s)
Javanmard, M. ; Dept. of Biochem., Stanford Univ., Stanford, CA, USA ; Davis, R.W.

In this letter, we present a scalable method based on the use of microfluidics and electrical conductivity measurement for monitoring the activity of an enzyme. This scalable method can be used for drug screening to test for the ability of an organic small molecule to inhibit the activity of an enzyme. Our method involves the use of electrodes integrated in a microchannel for the measurement of conductivity across the channel. An ac measurement is used to monitor the conductivity across the channel as the enzyme reaction proceeds. Here, we show the ability to measure these changes in conductivity and also the ability to distinguish between an active and inactive enzyme using an enzyme with two orders of magnitude reduced.

Published in:

Sensors Journal, IEEE  (Volume:12 ,  Issue: 9 )