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Instrumented indentation tests were conducted on a pure nickel and a Ni-10Pd solid solution at 450 °C with loading rates varying from 62.5 to 1000 mN/s. The load–depth curves from the pure nickel exhibited a smooth and continuous transition; while the load–depth curves from the Ni-10Pd were initially smooth and then became serrated after reaching a critical load. Increases in loading rates resulted in an earlier occurrence of the serrated flow with a higher load threshold. The mechanism responsible for the serration was delineated by accounting for the reconfiguration of dislocation substructures and the interactions between solutes and forest dislocations.