Cart (Loading....) | Create Account
Close category search window

Gene analysis of HeLa cells subjected to intense burst sinusoidal electric fields

Sign In

Cookies must be enabled to login.After enabling cookies , please use refresh or reload or ctrl+f5 on the browser for the login options.

Formats Non-Member Member
$31 $13
Learn how you can qualify for the best price for this item!
Become an IEEE Member or Subscribe to
IEEE Xplore for exclusive pricing!
close button

puzzle piece

IEEE membership options for an individual and IEEE Xplore subscriptions for an organization offer the most affordable access to essential journal articles, conference papers, standards, eBooks, and eLearning courses.

Learn more about:

IEEE membership

IEEE Xplore subscriptions

7 Author(s)
Yano, M. ; Grad. Sch. of Sci. & Technol., Kumamoto Univ., Kumamoto, Japan ; Matsumoto, C. ; Tanaka, N. ; Oide, T.
more authors

Intense pulsed electrics field are one of the agents to give physical stresses to biological systems which are the complex of dielectric materials. We have been using an intense burst sinusoidal electric field (IBSEF) as a narrow band field to discuss the biological effects of the field in the frequency domain. Our previous study demonstrated that the enhancement of proliferation activity of HeLa S3 cells is induced by the application of non-thermal, IBSEF with amplitudes of 300 kV/m and with frequencies between 3 and 100 MHz. Moreover, the application of the smaller number of the pulses enhanced the proliferation activity, whereas the cells were likely to be killed by the larger number of the pulses. According to calculation of the electric field distribution over the cell under AC field, the field strength of cell membrane is decreased with increasing the frequency in the range more than 1 MHz. The range of frequencies between 3-10 MHz, the distributed field strength on nuclear membrane became the maximum. This is because of the decrease in the electrical impedance of the membrane, which can be regarded as a dielectric film. The frequency dependence implies the proliferation activity might be associated with the electric field at the cell membrane and nuclear membrane. Here, we analyze expression of RNA gene related to cell growth including cell cycle and describe the mechanism of the enhancement of proliferation activity by means of reverse transcription polymerase chain reaction (RT-PCR) assays.

Published in:

Pulsed Power Conference (PPC), 2011 IEEE

Date of Conference:

19-23 June 2011

Need Help?

IEEE Advancing Technology for Humanity About IEEE Xplore | Contact | Help | Terms of Use | Nondiscrimination Policy | Site Map | Privacy & Opting Out of Cookies

A not-for-profit organization, IEEE is the world's largest professional association for the advancement of technology.
© Copyright 2014 IEEE - All rights reserved. Use of this web site signifies your agreement to the terms and conditions.