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We reports on a new patterning technique of conjugated polymer (i.e., PEDOT:PSS) with high conductivity which consists of UV-nanoimprinted surface modified substrate and second doping with ethylene glycol. Generally, organic conjugated polymers are very weak to standard photolithography because alcohol or high temperature treatment decreases conductivity of the polymers. Therefore, we proposed new fabrication process where firstly hydrophilic surface is patterned on plastic substrates with UV nano-imprint, PEDOT:PSS water-dispersion is patterned only on the hydrophilic area, then PEDOT:PSS is doped with ethylene glycol for increasing its conductivity. This process is just operated under room temperature and without alcohol and second doping is also produced, which lead to high conductivity of PEDOT:PSS.