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Hepatitis B surface antigen-antibody interactions studied by optical tweezers

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5 Author(s)
Zhou, Z.L. ; Dept. of Mech. Eng., Univ. of Hong Kong, Hong Kong, China ; Tang, B. ; Ngan, A.H.W. ; Dong, Z.N.
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The protein-protein interactions between hepatitis B surface antigen (HBsAg) and its antibodies (anti-HBs) were studied by measuring the binding force between microspheres coated with such proteins using optical tweezers. The interaction force between the protein-coated microspheres was found to be strongly influenced by the acidity of the surrounding liquid medium, as well as the experimental temperature, and it reaches a maximum value at around pH 7.5 and temperature around 37°C. By measuring the protein distribution on the surfaces of the microspheres and their contact areas using scanning electron microscopy, the specific binding force between an HBsAg and anti-HBs protein pair is estimated to be around 4.8-pN at the optimum pH value and temperature at an applied loading rate of around 1-pN/s.

Published in:

Nanobiotechnology, IET  (Volume:6 ,  Issue: 1 )