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Application of Artificial Immune System (AIS) evolves as a key Artificial Intelligence concept for detecting misbehavior, ensuring security, detecting faults and performing data mining in Mobile ad hoc Networks (MANETs). Recognition of misbehaving nodes is a must for proper functioning of a MANET. AIS approach has a unique feature of learning which is absent in other techniques (e.g. reputation system). Danger Signal and Clonal Selection are the key techniques of AIS for misbehavior detection. Different types of misbehavior occur in MANETs, and then at different network layers-Physical, Data Link, and Network. In this paper we investigate and detect misbehavior at Network Layer. We performed experiment using the concepts of danger signal and clonal selection of AIS. Result of the misbehavior detection system depends on the way we use the danger signal for misbehavior detection. We propose an enhancement in the misbehavior detection using proper handling of danger signal. We compare our proposed concept of misbehavior detection with existing concepts. We show the experimental results showing the improvement in performance of AIS for misbehavior detection.