By Topic

Quantum dots and Au nanoparticles conjugated fluorescent DNA probes into uniform microstructure by asymmetrical synthesis

Sign In

Cookies must be enabled to login.After enabling cookies , please use refresh or reload or ctrl+f5 on the browser for the login options.

Formats Non-Member Member
$33 $33
Learn how you can qualify for the best price for this item!
Become an IEEE Member or Subscribe to
IEEE Xplore for exclusive pricing!
close button

puzzle piece

IEEE membership options for an individual and IEEE Xplore subscriptions for an organization offer the most affordable access to essential journal articles, conference papers, standards, eBooks, and eLearning courses.

Learn more about:

IEEE membership

IEEE Xplore subscriptions

5 Author(s)
Zhao Dai ; State Key Laboratory of Hollow Fiber Membrane Materials and Processes, School of Environmental and Chemical Engineering, Tianjin Polytechnic University, Tianjin 300387, People's Republic of China ; Ying Li ; Wenjuan Guo ; Donglai Qi
more authors

The fluorescent DNA probes using CdTe quantum dots (QDs) and Au nanoparticles (AuNPs) had complicated microstructures because one QD or AuNP can be conjugated to more than one single-stranded DNA (ssDNA) on its surface. In this Letter, an asymmetrical synthesis method of DNA probes was presented to get the DNA probes into the uniform microstructure when poly(N-isopropylacrylamide-co-divinylbenzene) microspheres (PNDMs) were used as templates. The sterically restricted linkages of CdTe QDs with ssDNA (CdTe-DNA1) and AuNPs with complementary ssDNA (Au-DNA2) were both present on PNDMs. After the CdTe-DNA1 and Au-DNA2 were cleaved from carriers and hybridised with each other, the fluorescent DNA probes (CdTe-dsDNA-Au) were prepared using 1 QD with 1 AuNP microstructure and the corresponding quenching efficiency of probes was 64.8%.

Published in:

IET Micro & Nano Letters  (Volume:7 ,  Issue: 2 )