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The electrical properties of individual NiFe/Pt multilayer nanowires have been measured in situ by nanomanipulators in a scanning electron microscope. The electrical measurement of ∼50 nm diameter individual NiFe/Pt multilayer nanowires with polycrystalline microstructure shows that the nanowires have a resistivity of ∼2.2 × 10-7 Ω m (corresponding to a conductivity of ∼4.5× 106 Ω-1 m-1) and average resistance of individual NiFe-Pt interfaces of ∼0.2 Ω. The maximum failure current density of an individual NiFe/Pt nanowire was measured to be ∼9.63 × 1011 A m-2.