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In this research, we compare hydrothermally synthesized TiO2 nanorods in an unsealed and a sealed system, respectively. Due to the variation of hydrothermal concentration, the morphology is very different. Then we further pre-fabricate a TiO2 seed layer by the sol-gel method and then hydrothermally synthesize TiO2 nanorods. The TiO2 nanorods can also be grown on the TiO2 seed layer successfully. This result exhibits the promising potential for the growth of TiO2 nanorods on versatile substrates.