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We report the threshold voltage control and its application to electronic circuits of ink-jet printed organic thin-film transistors (OTFTs) with self-organized process using 6, 13-bis(triisopropylsilyl-ethynyl)pentacene (TIPS-pentacene) and insulating polymer on plastic substrate. The threshold voltage of OTFTs can be changed by using different polymer blending. The enhancement and depletion-mode OTFTs can be achieved, respectively, by using polystyrene and poly(vinylphenol) through self-organized blending on the common substrate. A unipolar depletion-load inverter composed of enhancement-mode driver and depletion-mode load OTFTs has been demonstrated with ink-jet printed TIPS-pentacene layer on both TFT channels. An inverter on plastic shows a gain of -90.8 V/V at the driving voltage of -2 V.