Referenced Items are not available for this document.
Highly sensitive and selective detection of morphine (MO) was realized by coupling an immunoreaction of an anti-MO monoclonal antibody and a surface-plasmon-resonance (SPR) phenomena. A normorphine-bovine serum albumin (NM-BSA) conjugate prepared as an antigen was immobilized on the Au thin film of sensor chip by physical adsorption. The incident angle of the SPR system using the NM-BSA immobilized chip increased almost linearly with increasing concentration of antibody up to ca. 5 μg/ml (ppm). The addition of MO into the antibody solution (5 ppm) was found to reduce the incident angle shift sharply because of the inhibition effect of MO. Based on this inhibiting principle, the present sensor could detect MO very sensitively in the concentration range of 0.1-10 ng/ml (ppb)
Published in:
Solid State Sensors and Actuators, 1997. TRANSDUCERS '97 Chicago., 1997 International Conference on
(Volume:1
)
Date of Conference: 16-19 Jun 1997
- Page(s):
- 179 - 182 vol.1
- Meeting Date :
- 16 Jun 1997-19 Jun 1997
- Print ISBN:
- 0-7803-3829-4
- INSPEC Accession Number:
- 5744642
- Conference Location :
- Chicago, IL
- Digital Object Identifier :
- 10.1109/SENSOR.1997.613612
- Product Type:
- Conference Publications
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