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Optimization of medium components for low-temperature-active phytase production by Kurthia CZC0806 using response surface methodology

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2 Author(s)
Shuang Yu ; Sch. of Life Sci. & Biotechnol., Dalian Univ., Dalian, China ; Qingfang Zhang

Response surface methodology (RSM) was taken to enhance the production of low-temperature-active phytase from the strain Kurthia CZC0806 which was isolated from the Yellow Sea in China. The Plackett-Burman and Box-Behnken design were applied to optimize the fermentation medium components of the strain. Peptone, saccharose and NaCl were selected as the most significant factors, based on the Plackett-Burman design. The Box-Behnken was designed for these three factors. The results were analyzed by using the Minitabl5.0 soft. The optimal medium is: Peptone, saccharose and NaCl (at 20°C, initial pH 7.0). In the optimal medium conditions, the activity of the enzyme is 103.11 U/mL.

Published in:

IT in Medicine and Education (ITME), 2011 International Symposium on  (Volume:2 )

Date of Conference:

9-11 Dec. 2011