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Objective: To identify the thin layer of quercetin in Berchemia lineata and set up its content determination method. Methodology: To identify the quercetin in Berchemia lineate by adopting TLC (thin-layer chromatography); and to determine the content by HPLC (high performance liquid chromatography). The chromatographic column is Diamonsil ODS Cm (4.6 mm × 250 mm, 5 μ m) and the mobile phase is acetonitrile - methanol (5:1) A: 0.1% phosphoric acid solution B (Gradient: 0-30min, 20-60%A). The flow velocity, detection wave length and the column temperature are 0.85mL/min, 360nm and 30 °C respectively. Results: TLC (thin-layer chromatography) can identify the quercetin. The quercetin shows a good linearity within the range of 0.69 μ g~4.83 μ g, r=0.9998(n=7) and the average recovery rate is 99.8% (RSD: 1.27%, n=6). Conclusion: This method is simple, convenient and accurate and it could be used to control the quality of Berchemia lineate medicinal materials.