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This paper focuses on an ultra-sensitive H2 sensor fabricated using a time and cost-effective method. The sensor is comprised of Pd nanowires fabricated using λ-DNA templates. The morphology and the electrical characterisation of the metalized DNA nanowires (diameter = 5-45nm) were investigated by AFM and conductive AFM (cAFM). The conductivity of a single Pd-DNA nanowire, 127 Scm-1 was substantially lower than that of bulk Pd (9.5×104 Scm-1). However, the sensor shows high sensitivity towards hydrogen evaluated by recording the change in the resistance on exposure to cycles of different concentrations of H2 in N2 flow at 330K. The sensor showed a reversible response to H2 concentrations between 2300 - 12500 ppm and a response time of ~85 s. These results indicate that using DNA as a template is a promising method for the fabrication of low cost and rapid response sensors.