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A dual-excitation difference technique for fluorescence rejection in Raman spectroscopy

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3 Author(s)
Zhijian Cai ; Inst. of Inf. Opt. Eng., Soochow Univ., Suzhou, China ; Wenlong Zou ; Jianhong Wu

Fluorescence disturbance is the primary limiting factor of biomedical Raman spectroscopy. This paper proposed a dual-excitation difference technique for fluorescence rejection. In this research, a low-cost dual-excitation system was built for Raman detection, and a robust deconvolution algorithm was developed for spectrum recovery. Simulation and experimental results demonstrated that this method could effectively eliminate the fluorescence background in Raman spectroscopy.

Published in:

Bioelectronics and Bioinformatics (ISBB), 2011 International Symposium on

Date of Conference:

3-5 Nov. 2011