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The tissue sample of lung cancer is imaged by phase contrast CT method based on synchrotron radiation in vitro, and the imaging results are reconstructed in three-dimensional limit. The experiments were performed at X-ray imaging and biomedical application beam-line (BL13W1) of Shanghai Synchrotron Radiation Facility (SSRF). The results show that lung cancer tissue has best imaging quality in the phase contrast imaging experiments. By the approach of filter back projection (FBP), the tomography images are obtained successfully. The large alveoli of lung cancer tissue are reconstructed by the surface rendering method. The reconstructed images can not only display the internal structures of lung cancer tissues, but also show lung cancer cells eroded. Therefore, the phase contrast CT imaging based on synchrotron radiation is expected to become a means of observing the details of tumor tissue.