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Label-free electrical detection of surface DNA immobilization and hybridization via streaming current measurements in a microchannel is demonstrated. Streaming currents generated by the flow of deionised water through a polydimethysiloxane microchannel sealed on glass are measured using integrated Au electrodes and are sensitive to the density and polarity of the charge on the channel surface. An in-channel DNA hybridization protocol was developed. Streaming currents were monitored after each of protocol steps. The technique was applied to label free recognition of DNA hybridization and could distinguish between assays with complementary and non-complementary DNA strands.