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Observation of electric-field induced aggregation in crystallizing protein solutions by forward light scattering

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3 Author(s)
Wakamatsu, Takashi ; Department of Electrical and Electronics System Engineering, Ibaraki National College of Technology, Hitachinaka, Ibaraki 312-8508, Japan ; Toyoshima, Susumu ; Shimizu, Hidehiko

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Protein aggregation has been observed in-situ in lysozyme solutions under an internal electric field using a forward light scattering technique. Light scattering at small forward angles revealed that the application of a low voltage to the protein solutions significantly promotes aggregate formation. The solutions required a specific amount of time exposed to an electric field before the solution had undergone sufficient protein aggregation. The in-situ results from forward light scattering allow the characterization of the process.

Published in:

Applied Physics Letters  (Volume:99 ,  Issue: 15 )

Date of Publication:

Oct 2011

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