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High-Speed Three-Dimensional Microscope for Dynamically Moving Biological Objects Based on Parallel Phase-Shifting Digital Holographic Microscopy

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10 Author(s)
Tahara, T. ; Grad. Sch. of Sci. & Technol., Kyoto Inst. of Technol., Kyoto, Japan ; Yonesaka, R. ; Yamamoto, S. ; Kakue, T.
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We succeeded in 3-D motion-picture imaging for dynamic motion of biological objects swimming at different depths by parallel phase-shifting digital holographic microscopy (PPSDHM). PPSDHM is a technique for high-quality instantaneous 3-D imaging of specimens in the microscopic field of view by single-shot recording of multiple phase-shifted holograms. We constructed a PPSDHM for recording high-speed dynamic phenomena and then motions of biological objects in water were recorded at 20 000 and 150 000 frames/s, respectively, which is the fastest among the previous reports on 3-D imaging of biological objects.

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Selected Topics in Quantum Electronics, IEEE Journal of  (Volume:18 ,  Issue: 4 )