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Effects of pulsed electric fields on dna synthesis in an osteoblast-like cell line UMR-I06

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5 Author(s)
Long, Ying ; Department of Electrical Engineering Tsinghua University Beijing 100084 China ; Liu, Hong ; Guan, Zhicheng ; He, Xiaower
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The study of the bioeffects of electromagnetic fields EMFs is an important national task in biological physics. Using EMFs to treat bone diseases involves electrical technology biology and medicine. But the effects of EMFs are still controversial and the mechanisms are not yet clear. Therefore more effect is needed to detect the effects at the cellular and molecular levels. This paper investigates the effects of low-energy low-frequency pulsed capacitively coupled electric fields PCCEFs on DNA synthesis in UMR-106 osteoblast-like cells. The equipment can generate 25–250 Hz frequency 0–300 V amplitude and 0.2 ms pulse width signal. DNA synthesis is judged by the uptake of 3H-thymidine 3H-TdR. The results showed that the response of UMR-106 cells to electric field exposure are characterized by a a frequency window for increased DNA synthesis with a peak near 125 Hz b decreased synthesis with increasing electric intensity with repression at 100 V/cm and 25 Hz.

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Tsinghua Science and Technology  (Volume:5 ,  Issue: 4 )