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This letter proposes a power delay profile (PDP) estimation technique for linear minimum mean square error (LMMSE) channel estimator of multiple-input multiple-output orthogonal frequency division multiplexing (MIMO-OFDM) systems. For practical applications, only the pilot symbols of all transmit antenna ports are used in estimating the PDP. The distortions caused by null subcarriers and an insufficient number of samples for PDP estimation are also considered. The proposed technique effectively reduces the distortions for accurate PDP estimation. Simulation results show that the performance of LMMSE channel estimation using the proposed PDP estimate approaches that of Wiener filtering due to the mitigation of distortion effects.