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Intracellular water volume measurement using NMR

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2 Author(s)
Riqing, Zhang ; Department of Biological Science and Biotechnology, State Key Laboratory of Biomembrane and Membrane Biotechnology, Tsinghua University, Beijing 100084 ; Nanming, Zhao

Intracellular water volume is an important pararreter for expressing corcentration of intracell ular ions and diffusional water of rreabiby of cells by nuclear magnetic resonarce (NMR). Presented here, is a method using 35CI and deuterium (2D) NMR spectra of cell-free supernatant and cell suspension to get intracellui ar water volurre. The vol of intracell cell water as a fraction of the total cell volurre, Vi/Vc-in isosmotic solution is 0. 706 0.065.

Published in:

Tsinghua Science and Technology  (Volume:2 ,  Issue: 1 )