Intracellular water volume measurement using NMR

Intracellular water volume is an important pararreter for expressing corcentration of intracell ular ions and diffusional water of rreabiby of cells by nuclear magnetic resonarce (NMR). Presented here, is a method using
³⁵
CI and deuterium (
²
D) NMR spectra of cell-free supernatant and cell suspension to get intracellui ar water volurre. The vol of intracell cell water as a fraction of the total cell volurre, V
i
/V
c
-in isosmotic solution is 0. 706 0.065.