Effects of cholate on the stability of bacteriorhodopsin in alkaline solution

The effects of cholate on the structure of bacteriorhodopsin (bR) in the purple membrane and the effects of alkalion native and cholate-treated bR were studied using absorption spectra, fluorescence spectra and CD spectra. Treatment mass fraction of 10% cholate at room terrperature for 48 h had no obvious effect on the structure of bR, yet changed the composition of the purple merrbrane, making cholate-treated bR more stable in alkaline sol ution than native bR.