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Fermentation production of poly-β-hydroxybutyrate by recombinant escherichia coli

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3 Author(s)
Fang, Uu ; Department of Chemical Engineering, Tsinghua University, Beijing 100084 ; Hongqi, Li ; Zhongyao, Shen

Poly-β-hydroxybutyrate (PHB) was produced by recombinant E. coli HMS174 in a glucose-mineral medium. The medium composition and culture conditions were optimized by a series of shake flask experiments. The optimal initial concentrations of glucose and (NH4) 2SO4 were about 20 g/L and 4 g/L, respectively. The optimal temperature, pH, culture and inoculum volume were 37, 7.5, 25% and 10%, respectively. The final cell dry weight, PHB dry weight, PHB content and glucose conversion efficiency reached 8.06 g/L, 6.58 g/L, 81.64% and 98.56%, respectively, at 23.67 g/L initial glucose concentration after 48 h culturing. Different fed-batch cultures were also carried out in the shake flask.

Published in:

Tsinghua Science and Technology  (Volume:1 ,  Issue: 4 )