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Use of laser microbeam and ultrasound methods to enhance the efficiency of gene transfer into osteoblast cells

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6 Author(s)
Tang Qing ; Department of Biological Science and Biotechnology, State Key Laboratory of Biomembrane and Membrane Biotechnology, Tsinghua University, Beijing 100084 ; Chen Gang ; Shen Ziwei ; Zhang Zhicheng
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Gene tr ansfer introduced by physical methods is widely used for its convenient operation, high transfer efficiency and no host distinctive limitation. In this article pSV-β-Gal plasmid was induced into osteoblast cells successfully by laser microbeam method and ul trasound method, and β-Gal gene was highly expressed. The optimal conditions for the ultrasound and laser microbeam methods of gene transfer were obtained.

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Tsinghua Science and Technology  (Volume:3 ,  Issue: 3 )