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Interaction between proteins, cells and biomaterial surfaces is commonly observed and often used to measure biocoinpatibility of biomaterials. In this investigation, three kinds of biomaterials derived from chitosan were prepared. The surface wettability of these polymers, interaction of protein with material surface, and their effects on cell adhesion and growth were studied. The results show that the surface contact angle and surface charge of biomaterials have a close bearing on protein adsoiption as well as cell adhesion and growth, indicating that through different chemical modifications, chitosan can be made into different kinds of biomedical materials to satisfy various needs.