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Production of medium chain length polyhydroxyalkanoates by Pseudomonas mendocina 0806 from related and unrelated carbon sources

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7 Author(s)
Zhang, Guang ; Department of Biological Sciences and Biotechnology, Tsinghua University, Beijing 100084 ; Wu, Qiong ; Tian, Weidong ; Hong, Kui
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Pseudomonas mendocina strain 0806 isolated from oil-contaminated soil was found to produce medium chain length polyhydroxyalkanoates (mel PHAs). The mel PHAs consist of monomers with even numbers of carbon atoms such as hydroxyhexanoate (HHx or C6), hydroxyoctanoate (HO or C8), and/or hydroxydecanoate (HD or C10) as major compositions when the strain was grown on unrelated carbon sources such as glucose, citric acid and related carbon sources such as octanoate, myristic acid or oleic acid. While even and odd number hydroxyalkanoate (HA) monomers were synthesized when tridecanoic acid was used as carbon source. The molar ratio of carbon to nitrogen (RC/N) had strong effects on PHA compositions: the strain produced PHAs with 97%–99% (molar ratio) lID (C10) monomer when grown in a glucose anunonium sulfate medium of RC/N < 20; while 20% HO and 80% HD (molar fraction) were found as compositions in the polymers when growth was conducted in the media containing RC/N > 40. It was demonstrated that the molar ratio of HO/HD remained constant in the polymers synthesized from media containing a constant RC/N, regardless of the change of glucose concentration. Up to 3.6 g/L cell dry weight containing 45% (mass fraction) PHAs was produced by the strain grown for 48 h in a medium containing 25g/L glucose with RC/N of 40.

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Tsinghua Science and Technology  (Volume:4 ,  Issue: 3 )