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Biosensors are widely used in immunoassay. The biosensor chip carries a receptor which is used in immunoassay and the chip properties have an important influence on the detecting sensitivity of the biosensor. This paper describes a polystyrene based biosersor chip developed and used as part of a surface plasmon resonance (SPR) biosensor. The SPR biosensor has a much higher detecting senstivity than enzyme-linked immunoserbent assay (ELISA).