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An adaptive neural network output-feedback regulation approach is proposed for a class of multi-input-multi-output nonlinear time-varying delayed systems. Both the designed observer and controller are free from time delays. Different from the existing results, this paper need not the assumption that the upper bounding functions of time-delay terms are known, and only a neural network is employed to compensate for all the upper bounding functions of time-delay terms, so the designed controller procedure is more simplified. In addition, the resulting closed-loop system is proved to be semi-globally ultimately uniformly bounded, and the output regulation error converges to a small residual set around the origin. Two simulation examples are provided to verify the effectiveness of control scheme.