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The study on the impacting injure principle of blood in the high-speed axial flow blood pump was researched by using the theory of hemorheology and stress wave. The fragmentation process and impacting parameters and conditions of impacting between red blood cell and solid wall are analyzed. Through the observation analysis on the blood living performance and red blood cell micro shapes at several impacting speeds, the critical impact speed on the red blood cell fragmentation was obtained by blood impacting experiment. The research results indicate that red blood cell may be broken to lead hemolysis if the perpendicular impact speed is larger than 6 m/s.