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Radio Frequency Identification (RFID) systems, due to recent technological advances, have been used for various advantages in industry like production facilities, supply chain management etc. However, sometimes this requires a dense deployment of readers to cover the working area. Without optimizing reader's location and number, many of them will be redundant, reducing the efficiency of the whole RFID system. There are many algorithms proposed to solve this redundant reader problem, but all existing algorithms are based on omnidirectional reader antenna pattern, which is not practical. In this paper, a genetic algorithm is used to optimize the antenna beam to eliminate redundant reader based on real directional reader antenna pattern.