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The relationship between exposure to electromagnetic fields (EMF) and health risks is of increasing interest. Although general concern regarding the potential hazards of exposure to an EMF has led to many epidemiological investigations, the effects of EMF exposure on human cells are still controversial. In vitro study, the cellular immune effects of EMF are discussed. We investigated the effects of a high-frequency electromagnetic field (HFEMF) at 2.45 GHz on human interleukin 1β (IL-1β) and interleukin 6 (IL-6) productions in human monoblastic U937 cells. Our results showed that the exposure to a HFEMF of 2.45 GHz at SAR of 1 and 10 W/kg for 4 hours has no significant effect on IL-1β and IL-6 productions in U937 cells.