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The automotive industry is migrating towards all-electric and plug-in hybrid electric vehicles. Nevertheless conventional energy, in the form of thermal power and nuclear power, are used to power these vehicles which are not green and clean. The photosynthetic electrochemical cell (PEC) is one of the alternative energy resources for EVs and PHEVs. It could prove to be the ideal replacement for photovoltaic based charging applications. PEC uses aerobic microorganisms to produce energy. PEC produces energy under both light and dark conditions unlike photovoltaic cell. This paper presents, the operation of the PEC, voltage versus current characteristic, effect of anolyte and catholyte concentration.