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In this paper, we present a study that deals with response time of Networked Automation Systems (NAS). The main aim is to verify experimentally the validity of an analytic formulation of NAS response time, obtained in our past investigations . To that purpose, we carried out, under different conditions, a lot of measurements on a laboratory facility. Each time, we compared the observed measures to the predicted values. Overall, the study reveals clear agreement between the real observations and the theoretic predictions of our formula. It therefore promotes the formula to be used confidently for NAS response time evaluation while avoiding tedious and onerous experiments.